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goat ant mosm  (R&D Systems)


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    Structured Review

    R&D Systems goat ant mosm
    Goat Ant Mosm, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goat+anti+osm/Mouse+Oncostatin+M%2FOSM+Antibody/pm37688912-208-79-87
    Average 93 stars, based on 31 article reviews
    goat ant mosm - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Recombinant:

    Article Title: Helicobacter pylori outer membrane vesicles induce expression and secretion of oncostatin M in AGS gastric cancer cells.
    Article Snippet: Goat anti-OSMRβ (N-20) and all HRP-conjugated secondary antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, USA). .. Recombinant human OSM protein (295- OM), goat anti-OSM (AF-295-NA), and goat anti-GAPDH were from R&D systems (Minneapolis, USA). .. Polymyxin B (P4932), fetal bovine serum (FBS), penicillin/streptomycin (P/S), RPMI 1640 cell culture media, bovine serum albumin (BSA), phosphate-buffered saline (PBS), puromycin, and protease inhibitor cocktail (PIC) were from Sigma (St. Quentin Fallavier, France).

    Activity Assay:

    Article Title: Oncostatin M expression in the mouse uterus during early pregnancy promotes embryo implantation and decidualization.
    Article Snippet: .. Antigen recovery was performed in 0.01 M sodium citrate buffer (pH 6.0) by microwaving for 10 min. Endogenous horseradish peroxidase (HRP) activity was treated with 3% H2O2 for 15 min. After blocking with 10% horse serum for 1 h at 37 °C, sections were incubated with goat anti-Osm (0.5 μg/ml, R&D systems, #AF-495-NA, Minneapolis, MN) at 4 °C overnight. .. After washing, the sections were incubated with biotin-labeled rabbit anti-goat IgG antibody (Zhongshan Golden Bridge, Beijing, China) and streptavidin-HRP complex (Zhongshan Golden Bridge, Beijing, China).

    Blocking Assay:

    Article Title: Oncostatin M expression in the mouse uterus during early pregnancy promotes embryo implantation and decidualization.
    Article Snippet: .. Antigen recovery was performed in 0.01 M sodium citrate buffer (pH 6.0) by microwaving for 10 min. Endogenous horseradish peroxidase (HRP) activity was treated with 3% H2O2 for 15 min. After blocking with 10% horse serum for 1 h at 37 °C, sections were incubated with goat anti-Osm (0.5 μg/ml, R&D systems, #AF-495-NA, Minneapolis, MN) at 4 °C overnight. .. After washing, the sections were incubated with biotin-labeled rabbit anti-goat IgG antibody (Zhongshan Golden Bridge, Beijing, China) and streptavidin-HRP complex (Zhongshan Golden Bridge, Beijing, China).

    Incubation:

    Article Title: Oncostatin M expression in the mouse uterus during early pregnancy promotes embryo implantation and decidualization.
    Article Snippet: .. Antigen recovery was performed in 0.01 M sodium citrate buffer (pH 6.0) by microwaving for 10 min. Endogenous horseradish peroxidase (HRP) activity was treated with 3% H2O2 for 15 min. After blocking with 10% horse serum for 1 h at 37 °C, sections were incubated with goat anti-Osm (0.5 μg/ml, R&D systems, #AF-495-NA, Minneapolis, MN) at 4 °C overnight. .. After washing, the sections were incubated with biotin-labeled rabbit anti-goat IgG antibody (Zhongshan Golden Bridge, Beijing, China) and streptavidin-HRP complex (Zhongshan Golden Bridge, Beijing, China).



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    Image Search Results


    (A) Mouse primary myotubes were treated with recombinant OSM (250 ng/ml) and IgG or anti-OSM antibodies (10 μg/ml) for 48 hr. Changes in gene expression were determined by RT-qPCR (n = 3 for each group). ** p < 0.01, *** p < 0.001 compares differences between IgG and IgG + OSM groups. # p < 0.05, ## p < 0.01, ### p < 0.001 compares differences between IgG + OSM and anti-OSM + OSM groups. (B-H) Mice inoculated with LLC cells received IgG or anti-OSM antibody injections and were sacrificed 16 days post-tumor inoculation. Tumor (C) and muscle tissues (D) were weighed. Forelimb grip strength was measured before the sacrifice (n = 5 for WT-Control group and n = 6 for other groups) (E). Gastrocnemius muscle cross-sections were H&E stained (G), cross-sectional area (CSA) (F) and the fiber frequency distribution (H) were measured (n = 3 for each group). The scale bar is 100 μm. (I-J) Gastrocnemius muscle protein levels were determined by western blotting. Asterisk (*) indicates non-specific band (G). Band intensities were quantified (J) (n = 3 for each group). * p < 0.05, ** p < 0.01, compares differences between Control-IgG and Tumor-IgG groups. # p < 0.05, compares differences between Tumor-IgG and Tumor-anti-OSM groups. Data are presented as individual measurements (points) and mean ± SEM. Statistical analysis was conducted using one-way ANOVA.

    Journal: bioRxiv

    Article Title: Activated Oncostatin M signaling drives cancer-associated skeletal muscle wasting

    doi: 10.1101/2023.01.26.525658

    Figure Lengend Snippet: (A) Mouse primary myotubes were treated with recombinant OSM (250 ng/ml) and IgG or anti-OSM antibodies (10 μg/ml) for 48 hr. Changes in gene expression were determined by RT-qPCR (n = 3 for each group). ** p < 0.01, *** p < 0.001 compares differences between IgG and IgG + OSM groups. # p < 0.05, ## p < 0.01, ### p < 0.001 compares differences between IgG + OSM and anti-OSM + OSM groups. (B-H) Mice inoculated with LLC cells received IgG or anti-OSM antibody injections and were sacrificed 16 days post-tumor inoculation. Tumor (C) and muscle tissues (D) were weighed. Forelimb grip strength was measured before the sacrifice (n = 5 for WT-Control group and n = 6 for other groups) (E). Gastrocnemius muscle cross-sections were H&E stained (G), cross-sectional area (CSA) (F) and the fiber frequency distribution (H) were measured (n = 3 for each group). The scale bar is 100 μm. (I-J) Gastrocnemius muscle protein levels were determined by western blotting. Asterisk (*) indicates non-specific band (G). Band intensities were quantified (J) (n = 3 for each group). * p < 0.05, ** p < 0.01, compares differences between Control-IgG and Tumor-IgG groups. # p < 0.05, compares differences between Tumor-IgG and Tumor-anti-OSM groups. Data are presented as individual measurements (points) and mean ± SEM. Statistical analysis was conducted using one-way ANOVA.

    Article Snippet: Mouse OSM antibody (AF-495-NA) and control goat IgG antibody (AB-108-C) were purchased from R&D Systems.

    Techniques: Recombinant, Gene Expression, Quantitative RT-PCR, Control, Staining, Western Blot

    Species-specific binding differences of LIF and OSM to OSMR and LIFR in human and mice. ( A ) Schematic illustration of species-specific binding properties of human OSM (hOSM), human LIF (hLIF), murine OSM (mOSM) and murine LIF (mLIF). Note that the generation of a human-like OSM (hlOSM) mutant mimics the binding properties of hOSM with the OSMR and LIFR in mice . ( B ) Three-dimensional model of mOSM, mLIF and hlOSM. The AB loop and D–helix of each molecule constitute structural determinants of their species-specific receptor binding properties. The hlOSM protein contains the human AB loop sequence, which is highlighted in green.

    Journal: International Journal of Molecular Sciences

    Article Title: Concomitant Activation of OSM and LIF Receptor by a Dual-Specific hlOSM Variant Confers Cardioprotection after Myocardial Infarction in Mice

    doi: 10.3390/ijms23010353

    Figure Lengend Snippet: Species-specific binding differences of LIF and OSM to OSMR and LIFR in human and mice. ( A ) Schematic illustration of species-specific binding properties of human OSM (hOSM), human LIF (hLIF), murine OSM (mOSM) and murine LIF (mLIF). Note that the generation of a human-like OSM (hlOSM) mutant mimics the binding properties of hOSM with the OSMR and LIFR in mice . ( B ) Three-dimensional model of mOSM, mLIF and hlOSM. The AB loop and D–helix of each molecule constitute structural determinants of their species-specific receptor binding properties. The hlOSM protein contains the human AB loop sequence, which is highlighted in green.

    Article Snippet: Subsequently, membranes were probed with the following specific primary antibodies: goat anti-mouse OSMR (R&D Systems; Catalogue No. AF-662), goat anti-human LIFR (R&D Systems, Catalogue No. AF-249), goat anti-mouse OSM (R&D Systems, Catalogue No. AF-495), goat anti-mouse LIF (R&D Systems, Catalogue No. AF-449), rabbit anti-Phospho-Stat3 (Tyr705) (Cell Signaling, Catalogue No. 9131), rabbit anti-Stat3 (Cell Signaling, Catalogue No. 4904), rabbit anti-Phospho-Stat5 (Tyr694) (Cell Signaling, Catalogue No. 9359), rabbit anti-Stat5 (Cell Signaling, Catalogue No. 94205), rabbit anti-Phospho-c-Myc (Ser62) (Cell Signaling, Catalogue No. 13748) and rabbit anti c-Myc (Cell Signaling, Catalogue No. 5605).

    Techniques: Binding Assay, Mutagenesis, Sequencing

    Kinetic expression pattern of OSM, LIF and their corresponding receptors OSMR and LIFR in cardiac tissue after the onset of myocardial infarction in mice. ( A ) Schematic illustration of kinetic expression profiling. Cardiac tissue was harvested at indicated time points post-MI and fractionated into a non-infarcted remote zone (RZ) and infarction zone (IZ). Expression of OSMR, OSM, LIFR and LIF in RZ and IZ was analyzed by immunoblotting ( n = 6 at Day 1; n = 5 at Day 2; n = 7 at Day 4; n = 5 at Day 7; n = 5 at Day 14 post-MI). ( B ) Two representative immunoblots of OSMR, OSM, LIFR and LIF in RZ and IZ at indicated time points post-MI. ( C – F ) Semi-quantitative analysis of immunoblots shown in ( B ) based on the adjusted mean volume pixel density of bands. Statistical analysis was performed by two-way ANOVA. Asterisks indicate Bonferroni post-hoc test significances between RZ and IZ at individual time points as well as between RZ and IZ throughout the period of observation with * p < 0.05, ** p < 0.01, *** p < 0.001 and **** p < 0.0001. Data are presented as mean ± sem. Immunofluorescence analysis of ( G ) OSMR and ( H ) LIFR in infarcted hearts of mice 7 days after MI. F-actin: grey. OSMR: red. LIFR: blue. IZ: infarction zone. RZ: remote zone. RV: right ventricle. LV: left ventricle. Scale bars, 500 µm and 100 µm in magnified sections.

    Journal: International Journal of Molecular Sciences

    Article Title: Concomitant Activation of OSM and LIF Receptor by a Dual-Specific hlOSM Variant Confers Cardioprotection after Myocardial Infarction in Mice

    doi: 10.3390/ijms23010353

    Figure Lengend Snippet: Kinetic expression pattern of OSM, LIF and their corresponding receptors OSMR and LIFR in cardiac tissue after the onset of myocardial infarction in mice. ( A ) Schematic illustration of kinetic expression profiling. Cardiac tissue was harvested at indicated time points post-MI and fractionated into a non-infarcted remote zone (RZ) and infarction zone (IZ). Expression of OSMR, OSM, LIFR and LIF in RZ and IZ was analyzed by immunoblotting ( n = 6 at Day 1; n = 5 at Day 2; n = 7 at Day 4; n = 5 at Day 7; n = 5 at Day 14 post-MI). ( B ) Two representative immunoblots of OSMR, OSM, LIFR and LIF in RZ and IZ at indicated time points post-MI. ( C – F ) Semi-quantitative analysis of immunoblots shown in ( B ) based on the adjusted mean volume pixel density of bands. Statistical analysis was performed by two-way ANOVA. Asterisks indicate Bonferroni post-hoc test significances between RZ and IZ at individual time points as well as between RZ and IZ throughout the period of observation with * p < 0.05, ** p < 0.01, *** p < 0.001 and **** p < 0.0001. Data are presented as mean ± sem. Immunofluorescence analysis of ( G ) OSMR and ( H ) LIFR in infarcted hearts of mice 7 days after MI. F-actin: grey. OSMR: red. LIFR: blue. IZ: infarction zone. RZ: remote zone. RV: right ventricle. LV: left ventricle. Scale bars, 500 µm and 100 µm in magnified sections.

    Article Snippet: Subsequently, membranes were probed with the following specific primary antibodies: goat anti-mouse OSMR (R&D Systems; Catalogue No. AF-662), goat anti-human LIFR (R&D Systems, Catalogue No. AF-249), goat anti-mouse OSM (R&D Systems, Catalogue No. AF-495), goat anti-mouse LIF (R&D Systems, Catalogue No. AF-449), rabbit anti-Phospho-Stat3 (Tyr705) (Cell Signaling, Catalogue No. 9131), rabbit anti-Stat3 (Cell Signaling, Catalogue No. 4904), rabbit anti-Phospho-Stat5 (Tyr694) (Cell Signaling, Catalogue No. 9359), rabbit anti-Stat5 (Cell Signaling, Catalogue No. 94205), rabbit anti-Phospho-c-Myc (Ser62) (Cell Signaling, Catalogue No. 13748) and rabbit anti c-Myc (Cell Signaling, Catalogue No. 5605).

    Techniques: Expressing, Western Blot, Immunofluorescence